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Cellosaurus HUES8 iCas9 (CVCL_VR10)

[Text version]
Cell line name HUES8 iCas9
Accession CVCL_VR10
Resource Identification Initiative To cite this cell line use: HUES8 iCas9 (RRID:CVCL_VR10)
Comments From: Memorial Sloan Kettering Cancer Center; New York; USA.
Characteristics: Cell line with robust Dox-inducible expression of Cas9. Can be used for rapid and highly efficient generation of biallelic knockout hPSCs for loss-of-function studies.
Characteristics: Using TALEN two constructs SA-2A-Puro-Cas9-TRE and SA-2A-Neo-M2rtTA were introduced in the AAVS1 locus, one in each allele.
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_B207 (HUES 8)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
STR profile Source(s): MSKCC

Markers:
AmelogeninX,Y
CSF1PO10,12
D1S165615
D2S44111,15
D2S133817
D3S135815,19
D5S81811
D7S82011,12
D8S117913
D10S124814
D12S39116,18
D13S31712,14
D16S5399,12
D18S5112,16
D19S43313,15
D21S1128,29
D22S104514,15,16
DYS39110
FGA20,25
Penta D10,11
Penta E7,10
TH017,9
TPOX8,11
vWA15,19

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Publications

PubMed=24931489; DOI=10.1016/j.stem.2014.05.018
Gonzalez F., Zhu Z.-R., Shi Z.-D., Lelli K., Verma N., Li Q.V., Huangfu D.-W.
An iCRISPR platform for rapid, multiplexable, and inducible genome editing in human pluripotent stem cells.
Cell Stem Cell 15:215-226(2014)

Cross-references
Encyclopedic resources Wikidata; Q94308392
Entry history
Entry creation13-Nov-2018
Last entry update29-Jun-2023
Version number6