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Cellosaurus HEK293T GALE KO clone 4 (CVCL_VR11)

[Text version]
Cell line name HEK293T GALE KO clone 4
Accession CVCL_VR11
Resource Identification Initiative To cite this cell line use: HEK293T GALE KO clone 4 (RRID:CVCL_VR11)
Comments Characteristics: Devoid of all O-glycosylation. But with the addition of galactose and GalNAc to the culture media, salvage pathways are able to compensate for the lack of GALE enzymatic activity and restore O-linked glycosylation.
Knockout cell: Method=CRISPR/Cas9; HGNC; 4116; GALE.
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [Note=tsA].
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0063 (HEK293T)
Children:
CVCL_VR14 (HEK293T GALE+GALK1 KO clone 12)CVCL_VR15 (HEK293T GALE+GALK2 KO clone 7)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=28654657; DOI=10.1371/journal.pone.0179949
Termini J.M., Silver Z.A., Connor B., Antonopoulos A., Haslam S.M., Dell A., Desrosiers R.C.
HEK293T cell lines defective for O-linked glycosylation.
PLoS ONE 12:E0179949-E0179949(2017)

Cross-references
Encyclopedic resources Wikidata; Q94095755
Entry history
Entry creation13-Nov-2018
Last entry update05-Oct-2023
Version number7