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Cellosaurus HEK293-SREBP1-T2A-luciferase-KI (CVCL_VR42)

[Text version]

Cell line name HEK293-SREBP1-T2A-luciferase-KI
Accession CVCL_VR42
Resource Identification Initiative To cite this cell line use: HEK293-SREBP1-T2A-luciferase-KI (RRID:CVCL_VR42)
Comments Characteristics: Useful for studying transcriptional regulation of the SREBP1 gene and for screening drugs or chemical molecules that regulate SREBP1 gene expression (PubMed=30232659).
Characteristics: CRISPR/Cas9-mediated knock-in of a T2A-luciferase-CMV-eGFP-T2A-Neomycin construct at the C-terminal end of SREBP1.
Transfected with: UniProtKB; P08659; Firefly luciferase.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0045 (HEK293)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=30232659; DOI=10.1007/s10529-018-2608-2
Li Z.-H., Zhao J.-L., Muhammad N., Wang D.-Y., Mao Q.-W., Xia H.-B.
Establishment of a HEK293 cell line by CRISPR/Cas9-mediated luciferase knock-in to study transcriptional regulation of the human SREBP1 gene.
Biotechnol. Lett. 40:1495-1506(2018)

Cross-references
Other Wikidata; Q94095267
Entry history
Entry creation13-Nov-2018
Last entry update02-Jul-2020
Version number2