Cellosaurus A549 dCas9-KRAB (CVCL_VR73)
Cell line name | A549 dCas9-KRAB | ||||||||||||||||||
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Accession | CVCL_VR73 | ||||||||||||||||||
Resource Identification Initiative | To cite this cell line use: A549 dCas9-KRAB (RRID:CVCL_VR73) | ||||||||||||||||||
Comments | Population: Caucasian. Characteristics: Using CRISPR/Cas9 a construct containing KRAB-dCas9-2A-Pac-2A-RFP-PA was introduced in the AAVS1 safe harbor locus. Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB). Transfected with: UniProtKB; Q9U6Y8; Discosoma sp. red fluorescent protein drFP583 (DsRed, RFP). Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Lung; UBERON=UBERON_0002048. | ||||||||||||||||||
Sequence variations |
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Disease | Lung adenocarcinoma (NCIt: C3512) | ||||||||||||||||||
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) | ||||||||||||||||||
Hierarchy | Parent: CVCL_0023 (A-549) | ||||||||||||||||||
Sex of cell | Male | ||||||||||||||||||
Age at sampling | 58Y | ||||||||||||||||||
Category | Cancer cell line | ||||||||||||||||||
STR profile | Source(s): ATCC Markers:
Run an STR similarity search on this cell line | ||||||||||||||||||
Cross-references | |||||||||||||||||||
Cell line collections (Providers) | ATCC; CCL-185dCas9-KRAB | ||||||||||||||||||
Cell line databases/resources | cancercelllines; CVCL_VR73 | ||||||||||||||||||
Encyclopedic resources | Wikidata; Q93311895 | ||||||||||||||||||
Entry history | |||||||||||||||||||
Entry creation | 25-Feb-2019 | ||||||||||||||||||
Last entry update | 30-Jan-2024 | ||||||||||||||||||
Version number | 8 |