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Cellosaurus A549 dCas9-KRAB (CVCL_VR73)

[Text version]
Cell line name A549 dCas9-KRAB
Accession CVCL_VR73
Resource Identification Initiative To cite this cell line use: A549 dCas9-KRAB (RRID:CVCL_VR73)
Comments Population: Caucasian.
Characteristics: Using CRISPR/Cas9 a construct containing KRAB-dCas9-2A-Pac-2A-RFP-PA was introduced in the AAVS1 safe harbor locus.
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
Transfected with: UniProtKB; Q9U6Y8; Discosoma sp. red fluorescent protein drFP583 (DsRed, RFP).
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Sequence variations
  • Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
Disease Lung adenocarcinoma (NCIt: C3512)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0023 (A-549)
Sex of cell Male
Age at sampling 58Y
Category Cancer cell line
STR profile Source(s): ATCC

Markers:
AmelogeninX,Y
CSF1PO10,12
D5S81811
D7S8208,11
D13S31711
D16S53911,12,13
TH018,9.3
TPOX8,11
vWA14

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Cross-references
Cell line collections (Providers) ATCC; CCL-185dCas9-KRAB
Cell line databases/resources cancercelllines; CVCL_VR73
Encyclopedic resources Wikidata; Q93311895
Entry history
Entry creation25-Feb-2019
Last entry update30-Jan-2024
Version number8