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Cellosaurus U2OS#18 (CVCL_VR75)

[Text version]

Cell line name U2OS#18
Synonyms U2OS clone #18; U2OS reporter cell line #18
Accession CVCL_VR75
Resource Identification Initiative To cite this cell line use: U2OS#18 (RRID:CVCL_VR75)
Comments Characteristics: Contains a 'nested intron' reporter construct HRsub that allows to study sister chromatid recombination (SCR) by positive selection of 'long tract' gene conversion events between sister chromatids.
Doubling time: ~50-60 hours (DSMZ).
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
Transfected with: UniProtKB; P13249; S.alboniger pac.
Disease Osteosarcoma (NCIt: C9145)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
CVCL_VR78 (U2OS#18-RAD51B-8)CVCL_VR80 (U2OS#18-RAD51C-15)CVCL_VR82 (U2OS#18-RAD51D-4)
CVCL_VR84 (U2OS#18-XRCC2-5E)CVCL_VR86 (U2OS#18-XRCC3-6A)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line

PubMed=15590323; DOI=10.1016/j.dnarep.2004.08.010
Puget N., Knowlton M., Scully R.
Molecular analysis of sister chromatid recombination in mammalian cells.
DNA Repair 4:149-161(2005)

Cell line collections DSMZ; ACC-832
Entry history
Entry creation25-Feb-2019
Last entry updated25-Feb-2019
Version number1