ID   U2OS#24
AC   CVCL_VR87
SY   U2OS clone #24; U2OS reporter cell line #24
DR   Wikidata; Q98133787
RX   PubMed=15590323;
CC   Population: Caucasian.
CC   Characteristics: Contains a 'nested intron' reporter construct HRsub that allows to study sister chromatid recombination (SCR) by positive selection of 'long tract' gene conversion events between sister chromatids.
CC   Sequence variation: Mutation; HGNC; 9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0042 ! U2OS
SX   Female
AG   15Y
CA   Cancer cell line
DT   Created: 25-02-19; Last updated: 05-10-23; Version: 8
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RX   PubMed=15590323; DOI=10.1016/j.dnarep.2004.08.010;
RA   Puget N., Knowlton M., Scully R.;
RT   "Molecular analysis of sister chromatid recombination in mammalian
RT   cells.";
RL   DNA Repair 4:149-161(2005).
//