| Cell line name | E-PR293 |
|---|---|
| Accession | CVCL_VV75 |
| Resource Identification Initiative | To cite this cell line use: E-PR293 (RRID:CVCL_VV75) |
| Comments | Characteristics: Can be used to screen HIV-1 protease inhibitors. Produces HIV-1 PR fused to GFP in the presence of doxycycline. Characteristics: Transfected with the tetracycline transactivator (tTA) protein, a fusion of tetR with the activation domain of HSV-1 VP16. Transfected with: UniProtKB; P12947; HIV-1 isolate NY5 pol (PR). Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_V349 (HEK 293 Tet-Off) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Publications | PubMed=16815068; DOI=10.1016/j.micinf.2006.02.016 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q93533965 |
| Entry history | |
| Entry creation | 24-May-2019 |
| Last entry update | 05-Oct-2023 |
| Version number | 7 |