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Cellosaurus CHO-gmt4 (CVCL_W354)

[Text version]
Cell line name CHO-gmt4
Synonyms JW152
Accession CVCL_W354
Resource Identification Initiative To cite this cell line use: CHO-gmt4 (RRID:CVCL_W354)
Comments Group: Patented cell line.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); PTA-9657.
Characteristics: Lacks N-acetylglucosaminyltransferase I activity.
Selected for resistance to: UniProtKB; P02879; Ricinus communis ricin.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Mutation; UniProtKB; Q924C0; Mgat1; Simple; p.Gln339Ter (c.1015C>T); Zygosity=Unspecified (PubMed=20346410).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_0214 (CHO-K1)
Children:
CVCL_A8SP (CHO-gmt4D)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=20346410; DOI=10.1016/j.ymben.2010.03.002
Goh J.S.Y., Zhang P.-Q., Chan K.F., Lee M.M., Lim S.F., Song Z.-W.
RCA-I-resistant CHO mutant cells have dysfunctional GnT I and expression of normal GnT I in these mutants enhances sialylation of recombinant erythropoietin.
Metab. Eng. 12:360-368(2010)

CLPUB00638
Goh J.S.Y.
Applications of a novel CHO glycosylation mutant.
Thesis PhD (2014), National University of Singapore, Singapore

PubMed=24166780; DOI=10.1002/biot.201300301
Goh J.S.Y., Liu Y.-W., Liu H.-F., Chan K.F., Wan C., Teo G., Zhou X.-S., Xie F.-S., Zhang P.-Q., Zhang Y.-X., Song Z.-W.
Highly sialylated recombinant human erythropoietin production in large-scale perfusion bioreactor utilizing CHO-gmt4 (JW152) with restored GnT I function.
Biotechnol. J. 9:100-109(2014)

Patent=US9243235
Song Z.-W.
Method of producing recombinant proteins with mannose-terminated N-glycans.
Patent number US9243235, 26-Jan-2016

Cross-references
Encyclopedic resources Wikidata; Q54899194
Entry history
Entry creation16-Apr-2014
Last entry update30-Jan-2024
Version number11