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Cellosaurus JE6-1REP-iNKT (CVCL_WN97)

[Text version]
Cell line name JE6-1REP-iNKT
Accession CVCL_WN97
Resource Identification Initiative To cite this cell line use: JE6-1REP-iNKT (RRID:CVCL_WN97)
Comments Population: Caucasian.
Characteristics: Fluorescence-based iNKT-TCR reporter system, applicable for screening potential new lipid antigens that infuence iNKT cell activation.
Characteristics: The eGFP reporter gene is under control of a NF-kappaB promoter element.
Transfected with: HGNC; 12027; TRA (iNKT-TCR V-alpha24).
Transfected with: HGNC; 12155; TRB (iNKT-TCR V-beta11).
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: T-cell; CL=CL_0000084.
Sequence variations
  • Mutation; HGNC; 959; BAX; Simple; p.Glu41Argfs*19 (c.121delG); ClinVar=VCV000009512; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 959; BAX; Simple; p.Glu41Glyfs*33 (c.121dupG); ClinVar=VCV000009511; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 16712; FBXW7; Simple; p.Arg505Cys (c.1513C>T); ClinVar=VCV000069961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6079; INPP5D; Simple; p.Gln345Ter (c.1033C>T); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6079; INPP5D; Simple; c.1097+1065_1097+1112del47; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 7325; MSH2; Simple; p.Arg711Ter (c.2131C>T); ClinVar=VCV000090903; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 7329; MSH6; Simple; p.Phe1088Serfs*2 (c.3261delC); ClinVar=VCV000089363; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11491; SYK; Simple; p.Met34Hisfs*3 (c.98_99insG); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg196Ter (c.586C>T); ClinVar=VCV000043589; Zygosity=Heterozygous (from parent cell line).
Disease Childhood T acute lymphoblastic leukemia (NCIt: C7953)
Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_WN96 (Jurkat E6.1 NF-kappaB::eGFP)
Sex of cell Male
Age at sampling 14Y
Category Cancer cell line
Publications

PubMed=31092850; DOI=10.1038/s41598-019-43529-4
Humeniuk P., Geiselhart S., Battin C., Webb T.J., Steinberger P., Paster W., Hoffmann-Sommergruber K.
Generation of a Jurkat-based fluorescent reporter cell line to evaluate lipid antigen interaction with the human iNKT cell receptor.
Sci. Rep. 9:7426-7426(2019)

Cross-references
Cell line databases/resources cancercelllines; CVCL_WN97
Encyclopedic resources Wikidata; Q94327309
Entry history
Entry creation05-Jul-2019
Last entry update05-Oct-2023
Version number10