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Cellosaurus H1 iCas9 (CVCL_WS14)

[Text version]

Cell line name H1 iCas9
Accession CVCL_WS14
Resource Identification Initiative To cite this cell line use: H1 iCas9 (RRID:CVCL_WS14)
Comments From: Memorial Sloan Kettering Cancer Center; New York; USA.
Characteristics: Cell line with robust Dox-inducible expression of Cas9. Can be used for rapid and highly efficient generation of biallelic knockout hPSCs for loss-of-function studies. Using TALEN two constructs SA-2A-Hygro-Cas9-TRE and SA-2A-Neo-M2rtTA were introduced in the AAVS1 locus, one in each allele.
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Transfected with: UniProtKB; P00557; E.coli hygromycin-B 4-O-kinase (hph).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Miscellaneous: Direct author submission of STR profile from Huangfu D.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9771 (WA01)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
STR profile Source(s): Direct_author_submission

Markers:
AmelogeninX,Y
CSF1PO12,13
D2S133818,19
D3S135815
D5S8189,11
D7S8208,12
D8S117912,13
D13S3178,11
D16S5399,13
D18S5117,18
D19S43313,15
D21S1128,32.2
FGA20,24
TH019.3
TPOX8,11
vWA15,17

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Publications

PubMed=28196600; DOI=10.1016/j.stem.2017.01.001
Shi Z.-D., Lee K., Yang D., Amin S., Verma N., Li Q.V., Zhu Z., Soh C.-L., Kumar R., Evans T., Chen S., Huangfu D.
Genome editing in hPSCs reveals GATA6 haploinsufficiency and a genetic interaction with GATA4 in human pancreatic development.
Cell Stem Cell 20:675-688.e6(2017)

PubMed=31110351; DOI=10.1038/s41588-019-0408-9
Li Q.V., Dixon G., Verma N., Rosen B.P., Gordillo M., Luo R., Xu C., Wang Q., Soh C.-L., Yang D., Crespo M., Shukla A., Xiang Q., Dundar F., Zumbo P., Witkin M., Koche R., Betel D., Chen S., Massague J., Garippa R., Evans T., Beer M.A., Huangfu D.
Genome-scale screens identify JNK-JUN signaling as a barrier for pluripotency exit and endoderm differentiation.
Nat. Genet. 51:999-1010(2019)

Entry history
Entry creation05-Jul-2019
Last entry updated05-Jul-2019
Version number1