ID   H1 iCas9
AC   CVCL_WS14
DR   Wikidata; Q93935734
RX   PubMed=28196600;
RX   PubMed=31110351;
CC   From: Memorial Sloan Kettering Cancer Center; New York; USA.
CC   Characteristics: Cell line with robust Dox-inducible expression of Cas9. Can be used for rapid and highly efficient generation of biallelic knockout hPSCs for loss-of-function studies. Using TALEN two constructs SA-2A-Hygro-Cas9-TRE and SA-2A-Neo-M2rtTA were introduced in the AAVS1 locus, one in each allele.
CC   Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC   Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Miscellaneous: STR profile from personal communication of Huangfu D.
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
ST   Source(s): Direct_author_submission
ST   Amelogenin: X,Y
ST   CSF1PO: 12,13
ST   D13S317: 8,11
ST   D16S539: 9,13
ST   D18S51: 17,18
ST   D19S433: 13,15
ST   D21S11: 28,32.2
ST   D2S1338: 18,19
ST   D3S1358: 15
ST   D5S818: 9,11
ST   D7S820: 8,12
ST   D8S1179: 12,13
ST   FGA: 20,24
ST   TH01: 9.3
ST   TPOX: 8,11
ST   vWA: 15,17
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_9771 ! WA01
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 05-07-19; Last updated: 05-10-23; Version: 7
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RX   PubMed=28196600; DOI=10.1016/j.stem.2017.01.001;
RA   Shi Z.-D., Lee K., Yang D.-P., Amin S., Verma N., Li Q.V., Zhu Z.-R.,
RA   Soh C.-L., Kumar R., Evans T., Chen S.-B., Huangfu D.-W.;
RT   "Genome editing in hPSCs reveals GATA6 haploinsufficiency and a
RT   genetic interaction with GATA4 in human pancreatic development.";
RL   Cell Stem Cell 20:675-688.e6(2017).
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RX   PubMed=31110351; DOI=10.1038/s41588-019-0408-9;
RA   Li Q.V., Dixon G., Verma N., Rosen B.P., Gordillo M., Luo R.-H., Xu C.-L.,
RA   Wang Q., Soh C.-L., Yang D.-P., Crespo M., Shukla A., Xiang Q., Dundar F.,
RA   Zumbo P., Witkin M., Koche R., Betel D., Chen S.-B., Massague J.,
RA   Garippa R., Evans T., Beer M.A., Huangfu D.-W.;
RT   "Genome-scale screens identify JNK-JUN signaling as a barrier for
RT   pluripotency exit and endoderm differentiation.";
RL   Nat. Genet. 51:999-1010(2019).
//