ID   H1 iCas9
AC   CVCL_WS14
RX   PubMed=28196600;
RX   PubMed=31110351;
CC   From: Memorial Sloan Kettering Cancer Center; New York; USA.
CC   Characteristics: Cell line with robust Dox-inducible expression of Cas9. Can be used for rapid and highly efficient generation of biallelic knockout hPSCs for loss-of-function studies. Using TALEN two constructs SA-2A-Hygro-Cas9-TRE and SA-2A-Neo-M2rtTA were introduced in the AAVS1 locus, one in each allele.
CC   Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC   Transfected with: UniProtKB; P00557; E.coli hygromycin-B 4-O-kinase (hph).
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Miscellaneous: STR profile from personal communication of Huangfu D.
ST   Source(s): Direct_author_submission
ST   Amelogenin: X,Y
ST   CSF1PO: 12,13
ST   D13S317: 8,11
ST   D16S539: 9,13
ST   D18S51: 17,18
ST   D19S433: 13,15
ST   D21S11: 28,32.2
ST   D2S1338: 18,19
ST   D3S1358: 15
ST   D5S818: 9,11
ST   D7S820: 8,12
ST   D8S1179: 12,13
ST   FGA: 20,24
ST   TH01: 9.3
ST   TPOX: 8,11
ST   vWA: 15,17
OX   NCBI_TaxID=9606; ! Homo sapiens
HI   CVCL_9771 ! WA01
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 05-07-19; Last updated: 12-03-20; Version: 2
//
RX   PubMed=28196600; DOI=10.1016/j.stem.2017.01.001;
RA   Shi Z.-D., Lee K., Yang D., Amin S., Verma N., Li Q.V., Zhu Z.,
RA   Soh C.-L., Kumar R., Evans T., Chen S., Huangfu D.;
RT   "Genome editing in hPSCs reveals GATA6 haploinsufficiency and a
RT   genetic interaction with GATA4 in human pancreatic development.";
RL   Cell Stem Cell 20:675-688.e6(2017).
//
RX   PubMed=31110351; DOI=10.1038/s41588-019-0408-9;
RA   Li Q.V., Dixon G., Verma N., Rosen B.P., Gordillo M., Luo R., Xu C.,
RA   Wang Q., Soh C.-L., Yang D., Crespo M., Shukla A., Xiang Q., Dundar F.,
RA   Zumbo P., Witkin M., Koche R., Betel D., Chen S., Massague J.,
RA   Garippa R., Evans T., Beer M.A., Huangfu D.;
RT   "Genome-scale screens identify JNK-JUN signaling as a barrier for
RT   pluripotency exit and endoderm differentiation.";
RL   Nat. Genet. 51:999-1010(2019).
//