Home  |  Contact

Cellosaurus MHHi006-A-3 (CVCL_XJ45)

[Text version]
Cell line name MHHi006-A-3
Synonyms CFTRKO/CFTRdTomato89; HSC1285TiPS2_CFTRtomatoC89 (KO)
Accession CVCL_XJ45
Resource Identification Initiative To cite this cell line use: MHHi006-A-3 (RRID:CVCL_XJ45)
Comments From: Hannover Medical School, LEBAO (MHH); Hannover; Germany.
Characteristics: Using TALEN a ZeoR-T2A-dTomato(nuc) construct was integrated in one of the alleles of CFTR which has a 4 bp deletion in its Kozak sequence (PubMed=31080112).
Transfected with: tdTomato, a bright red fluorescent protein derived from drFP583 (DsRed).
Transfected with: UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_VN33 (MHHi006-A)
Sex of cell Male
Age at sampling <1D
Category Induced pluripotent stem cell
Publications

PubMed=31080112; DOI=10.1016/j.stemcr.2019.04.014
Merkert S., Schubert M., Olmer R., Engels L., Radetzki S., Veltman M., Scholte B.J., Zollner J., Pedemonte N., Galietta L.J.V., von Kries J.P., Martin U.
High-throughput screening for modulators of CFTR activity based on genetically engineered cystic fibrosis disease-specific iPSCs.
Stem Cell Reports 12:1389-1403(2019)

Cross-references
Cell line databases/resources hPSCreg; MHHi006-A-3
Encyclopedic resources Wikidata; Q95989465
Entry history
Entry creation19-Dec-2019
Last entry update29-Jun-2023
Version number5