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Cellosaurus CHO-K1 Ed-A1 (CVCL_YD18)

[Text version]
Cell line name CHO-K1 Ed-A1
Synonyms Ed-A1
Accession CVCL_YD18
Resource Identification Initiative To cite this cell line use: CHO-K1 Ed-A1 (RRID:CVCL_YD18)
Comments Characteristics: Has reduced Na+-dependent glutamate transport activity. Have an increased permeability to small neutral molecules.
Characteristics: Selection is effected by tritium suicide selection. Mutagenized cells are bathed in tritiated D-aspartate and stored so as to allow time for cells that had taken up the D-aspartate to be killed by radiation damage.
Selected for resistance to: NCIt; C165595; Tritium beta radiation.
Transformant: ChEBI; CHEBI:23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_0214 (CHO-K1)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=8101392; DOI=10.1007/BF01233071
Ash J.F., Igo R.P. Jr., Morgan M., Grey A.
Selection of Chinese hamster ovary cells (CHO-K1) with reduced glutamate and aspartate uptake.
Somat. Cell Mol. Genet. 19:231-243(1993)

PubMed=8782489; DOI=10.1007/BF02369900
Igo R.P. Jr., Ash J.F.
New mutations and phenotypes associated with glutamate and aspartate transport in Chinese hamster ovary (CHO-K1) cells.
Somat. Cell Mol. Genet. 22:87-103(1996)

PubMed=9075644; DOI=10.1007/s002329900195
Tong X., Ash J.F., Caldwell K.D.
Rapid swelling of a CHO-K1 aspartate/glutamate transport mutant in hypo-osmotic medium.
J. Membr. Biol. 156:131-139(1997)

Cross-references
Encyclopedic resources Wikidata; Q93451727
Entry history
Entry creation19-Dec-2019
Last entry update30-Jan-2024
Version number6