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Cellosaurus HEK293 CNR1 cAMP-Nomad (CVCL_YI91)

[Text version]

Cell line name HEK293 CNR1 cAMP-Nomad
Synonyms CB1 cAMP-Nomad; CB1 Nomad; cAMPNomad-CB1
Accession CVCL_YI91
Resource Identification Initiative To cite this cell line use: HEK293 CNR1 cAMP-Nomad (RRID:CVCL_YI91)
Comments Characteristics: In absence of cAMP, the fluorescent biosensor is localized in the cellular membrane. An increase in this second messenger concentration leads to change in the structural folding of Nomad biosensor that promotes its cellular relocation in the vesicular trafficking of the cells.
Characteristics: The red cAMP-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboFP650 fluorescent protein, a Ht31 peptide fused to a KDEL signal.
Transfected with: HGNC; 2159; CNR1.
Transfected with: UniProtKB; J9PBR7; Entacmaea quadricolor far-red fluorescent protein eqFP650.
Transfected with: UniProtKB; P00557; E.coli hygromycin-B 4-O-kinase (hph).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0045 (HEK293)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Web pages http://www.innoprot.com/en_productos.asp?idsf=68&id=17&idp=513
Cross-references
Other Wikidata; Q94092863
Entry history
Entry creation19-Dec-2019
Last entry update02-Jul-2020
Version number2