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Cellosaurus MHHi006-A-4 (CVCL_YJ73)

[Text version]

Cell line name MHHi006-A-4
Synonyms HSC1285iPS2_Nkx2.1eGFP_p63dTomato
Accession CVCL_YJ73
Resource Identification Initiative To cite this cell line use: MHHi006-A-4 (RRID:CVCL_YJ73)
Comments From: Hannover Medical School, LEBAO (MHH); Hannover; Germany.
Characteristics: Using TALEN a P2A-eGFP-T2A-PGK-hph construct was integrated in front of the stop codon of NXK2-1 and a P2A-dTomato-nucmem-T2A-NeoR_PGKPuroR construct was integrated into exon 10 of TP63.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: tdTomato; a bright red fluorescent protein derived from drFP583 (DsRed).
Transfected with: UniProtKB; P00557; E.coli hygromycin-B 4-O-kinase (hph).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from sampling site: Umbilical cord blood.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_WX23 (MHHi006-A-2)
Sex of cell Male
Age at sampling <1D
Category Induced pluripotent stem cell

PubMed=31791010; DOI=10.1016/j.scr.2019.101659
Drick N., Sahabian A., Pongpamorn P., Merkert S., Gohring G., Welte T., Martin U., Olmer R.
Generation of a NKX2.1 - p63 double transgenic knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-4).
Stem Cell Res. 42:101659-101659(2020)

Cell line databases/resources hPSCreg; MHHi006-A-4
Encyclopedic resources Wikidata; Q95989466
Entry history
Entry creation19-Dec-2019
Last entry update20-May-2021
Version number3