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Cellosaurus HEK293 CCKBR arrestin-Nomad (CVCL_YK05)

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Cell line name HEK293 CCKBR arrestin-Nomad
Synonyms CCK2 Arrestin-Nomad; ArrestNomad-CCKBR
Accession CVCL_YK05
Resource Identification Initiative To cite this cell line use: HEK293 CCKBR arrestin-Nomad (RRID:CVCL_YK05)
Comments Characteristics: In basal conditions, the arrestin Biosensor shows a very low fluorescence intensity. When an agonist binds to CCKBR, it is phosphorylated, promoting the recruitment of the arrestin-Nomad Biosensor and leading to a change in its structural folding and promoting an increase in fluorescence intensity.
Transfected with: HGNC; 1571; CCKBR.
Transfected with: HGNC; 712; ARRB2.
Transfected with: UniProtKB; Q6WV12; Pontellina plumata GFP2 (with p.Lys5Glu, p.Val117Met, p.Val149Asp, p.Val151Asp, p.Ala155Thr, p.Phe168Ser and p.Leu200Asp = TurboGFP).
Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0045 (HEK293)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Cross-references
Cell line collections (Providers) Innoprot; P70808
Encyclopedic resources Wikidata; Q94092845
Entry history
Entry creation19-Dec-2019
Last entry update05-Oct-2023
Version number9