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Cellosaurus HEK293 CCKBR calcium-Nomad (CVCL_YK06)

[Text version]

Cell line name HEK293 CCKBR calcium-Nomad
Synonyms CCKBR Ca2+Nomad; CCK2 Nomad
Accession CVCL_YK06
Resource Identification Initiative To cite this cell line use: HEK293 CCKBR calcium-Nomad (RRID:CVCL_YK06)
Comments Characteristics: In absence of calcium, the fluorescent biosensor is localized in the cellular membrane. An increase in this second messenger concentration leads to change in the structural folding of Nomad biosensor that promotes its cellular relocation in the vesicular trafficking of the cells.
Characteristics: The green calcium-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboGFP fluorescent protein, a calmodulin-binding peptide fused to a KDEL signal.
Transfected with: HGNC; 1571; CCKBR.
Transfected with: UniProtKB; Q6WV12; Pontellina plumata GFP2 (with p.Lys5Glu, p.Val117Met, p.Val149Asp, p.Val151Asp, p.Ala155Thr, p.Phe168Ser and p.Leu200Asp = TurboGFP).
Transfected with: UniProtKB; P00557; E.coli hygromycin-B 4-O-kinase (hph).
Transfected with: UniProtKB; P13249; S.alboniger pac.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0045 (HEK293)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Web pages http://www.innoprot.com/en_productos.asp?idsf=75&id=17&idp=421
Other Wikidata; Q94092849
Entry history
Entry creation19-Dec-2019
Last entry update02-Jul-2020
Version number2