| Cell line name | HEK293 CCKBR calcium-Nomad |
|---|---|
| Synonyms | CCKBR Ca2+Nomad; CCK2 Nomad |
| Accession | CVCL_YK06 |
| Resource Identification Initiative | To cite this cell line use: HEK293 CCKBR calcium-Nomad (RRID:CVCL_YK06) |
| Comments | Characteristics: In absence of calcium, the fluorescent biosensor is localized in the cellular membrane. An increase in this second messenger concentration leads to change in the structural folding of Nomad biosensor that promotes its cellular relocation in the vesicular trafficking of the cells. Characteristics: The green calcium-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboGFP fluorescent protein, a calmodulin-binding peptide fused to a KDEL signal. Transfected with: HGNC; 1571; CCKBR. Transfected with: UniProtKB; Q6WV12; Pontellina plumata GFP2 (with p.Lys5Glu, p.Val117Met, p.Val149Asp, p.Val151Asp, p.Ala155Thr, p.Phe168Ser and p.Leu200Asp = TurboGFP). Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0045 (HEK293) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Cross-references | |
| Cell line collections (Providers) | Innoprot; P70108 |
| Encyclopedic resources | Wikidata; Q94092849 |
| Entry history | |
| Entry creation | 19-Dec-2019 |
| Last entry update | 05-Oct-2023 |
| Version number | 9 |