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Cellosaurus HEK293 DRD1 cAMP-Nomad (CVCL_YK11)

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Cell line name HEK293 DRD1 cAMP-Nomad
Synonyms DRD1 cAMP-Nomad; DRD1 Nomad; cAMPNomad DRD1
Accession CVCL_YK11
Resource Identification Initiative To cite this cell line use: HEK293 DRD1 cAMP-Nomad (RRID:CVCL_YK11)
Comments Characteristics: In absence of cAMP, the fluorescent biosensor is localized in the cellular membrane. An increase in this second messenger concentration leads to change in the structural folding of Nomad biosensor that promotes its cellular relocation in the vesicular trafficking of the cells.
Characteristics: The green cAMP-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboGFP fluorescent protein, a Ht31 peptide fused to a KDEL signal.
Transfected with: HGNC; 3020; DRD1.
Transfected with: UniProtKB; Q6WV12; Pontellina plumata GFP2 (with p.Lys5Glu, p.Val117Met, p.Val149Asp, p.Val151Asp, p.Ala155Thr, p.Phe168Ser and p.Leu200Asp = TurboGFP).
Transfected with: UniProtKB; P00557; E.coli hygromycin-B 4-O-kinase (hph).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0045 (HEK293)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Web pages http://www.innoprot.com/en_productos.asp?idsf=68&id=17&idp=508
Cross-references
Other Wikidata; Q94093065
Entry history
Entry creation19-Dec-2019
Last entry update02-Jul-2020
Version number2