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Cellosaurus U2OS ADCYAP1R1 cAMP-Nomad (CVCL_YK25)

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Cell line name U2OS ADCYAP1R1 cAMP-Nomad
Synonyms PAC1R cAMPNomad; PAC1R Nomad; cAMP Nomad PAC1R; cAMPNomad-PACAPR
Accession CVCL_YK25
Resource Identification Initiative To cite this cell line use: U2OS ADCYAP1R1 cAMP-Nomad (RRID:CVCL_YK25)
Comments Characteristics: In absence of cAMP, the fluorescent biosensor is localized in the cellular membrane. An increase in this second messenger concentration leads to change in the structural folding of Nomad biosensor that promotes its cellular relocation in the vesicular trafficking of the cells.
Characteristics: The red cAMP-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboFP650 fluorescent protein, a Ht31 peptide fused to a KDEL signal.
Transfected with: HGNC; 242; ADCYAP1R1.
Transfected with: UniProtKB; J9PBR7; Entacmaea quadricolor far-red fluorescent protein eqFP650.
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transfected with: UniProtKB; P13249; S.alboniger pac.
Derived from sampling site: Bone; tibia.
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Web pages http://www.innoprot.com/en_productos.asp?idsf=68&id=17&idp=494
Entry history
Entry creation19-Dec-2019
Last entry update12-Mar-2020
Version number2