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Cellosaurus U2OS CCKAR DAG-Nomad (CVCL_YK34)

Cell line name U2OS CCKAR DAG-Nomad
Synonyms DAG-NOMAD CCKAR; DAGNomad-CCKAR; CCKAR DAGNomad
Accession CVCL_YK34
Resource Identification Initiative To cite this cell line use: U2OS CCKAR DAG-Nomad (RRID:CVCL_YK34)
Comments Population: Caucasian.
Characteristics: In absence of DAG, the fluorescent biosensor is localized in the cellular membrane. An increase in this second messenger concentration leads to change in the structural folding of Nomad biosensor that promotes its cellular relocation in the vesicular trafficking of the cells.
Transfected with: HGNC; 1570; CCKAR.
Transfected with: UniProtKB; J9PBR7; Entacmaea quadricolor far-red fluorescent protein eqFP650.
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations
  • Mutation; HGNC; 9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) Innoprot; P70601
Encyclopedic resources Wikidata; Q98133731
Entry history
Entry creation19-Dec-2019
Last entry update05-Oct-2023
Version number9