| Cell line name | U2OS CHRM4 PKA-Nomad |
|---|---|
| Synonyms | PKA-Nomad-CHRM4; PKANomad-CHRM4; M4 Nomad; CHRM4 PKANomad |
| Accession | CVCL_YK38 |
| Resource Identification Initiative | To cite this cell line use: U2OS CHRM4 PKA-Nomad (RRID:CVCL_YK38) |
| Comments | Population: Caucasian. Characteristics: Before PKA activation, the fluorescent biosensor is localized in the cellular membrane. After receptor activation, PKA is activated and this activated PKA leads to change in the structural folding of Nomad Biosensor that promotes its cellular relocation in the vesicular trafficking of the cells. Transfected with: HGNC; 1953; CHRM4. Transfected with: UniProtKB; Q6WV12; Pontellina plumata GFP2 (with p.Lys5Glu, p.Val117Met, p.Val149Asp, p.Val151Asp, p.Ala155Thr, p.Phe168Ser and p.Leu200Asp = TurboGFP). Transfected with: UniProtKB; P00552; Transposon Tn5 neo. Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979. |
| Sequence variations |
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| Disease | Osteosarcoma (NCIt: C9145) Osteosarcoma (ORDO: Orphanet_668) |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0042 (U2OS) |
| Sex of cell | Female |
| Age at sampling | 15Y |
| Category | Cancer cell line |
| Cross-references | |
| Cell line collections (Providers) | Innoprot; P70545 |
| Encyclopedic resources | Wikidata; Q98133738 |
| Entry history | |
| Entry creation | 19-Dec-2019 |
| Last entry update | 05-Oct-2023 |
| Version number | 9 |