ID   U2OS CHRM4 PKA-Nomad
AC   CVCL_YK38
SY   PKA-Nomad-CHRM4; PKANomad-CHRM4; M4 Nomad; CHRM4 PKANomad
DR   Innoprot; P70545
DR   Wikidata; Q98133738
CC   Population: Caucasian.
CC   Characteristics: Before PKA activation, the fluorescent biosensor is localized in the cellular membrane. After receptor activation, PKA is activated and this activated PKA leads to change in the structural folding of Nomad Biosensor that promotes its cellular relocation in the vesicular trafficking of the cells.
CC   Sequence variation: Mutation; HGNC; 9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
CC   Transfected with: HGNC; 1953; CHRM4.
CC   Transfected with: UniProtKB; Q6WV12; Pontellina plumata GFP2 (with p.Lys5Glu, p.Val117Met, p.Val149Asp, p.Val151Asp, p.Ala155Thr, p.Phe168Ser and p.Leu200Asp = TurboGFP).
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0042 ! U2OS
SX   Female
AG   15Y
CA   Cancer cell line
DT   Created: 19-12-19; Last updated: 05-10-23; Version: 9
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