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Cellosaurus U2OS CHRM5 calcium-Nomad (CVCL_YK39)

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Cell line name U2OS CHRM5 calcium-Nomad
Synonyms Ca++ Nomad M5 (CHRM5)/U2OS; M5 Nomad
Accession CVCL_YK39
Resource Identification Initiative To cite this cell line use: U2OS CHRM5 calcium-Nomad (RRID:CVCL_YK39)
Comments Characteristics: In absence of calcium, the fluorescent biosensor is localized in the cellular membrane. An increase in this second messenger concentration leads to change in the structural folding of Nomad biosensor that promotes its cellular relocation in the vesicular trafficking of the cells.
Characteristics: The green calcium-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboGFP fluorescent protein, a calmodulin-binding peptide fused to a KDEL signal.
Transfected with: HGNC; 1954; CHRM5.
Transfected with: UniProtKB; Q6WV12; Pontellina plumata GFP2 (with p.Lys5Glu, p.Val117Met, p.Val149Asp, p.Val151Asp, p.Ala155Thr, p.Phe168Ser and p.Leu200Asp = TurboGFP).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transfected with: UniProtKB; P13249; S.alboniger pac.
Derived from sampling site: Bone; tibia.
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Web pages http://www.innoprot.com/en_productos.asp?idsf=75&id=17&idp=409
Entry history
Entry creation19-Dec-2019
Last entry update12-Mar-2020
Version number2