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Cellosaurus U2OS MPX-Nomad (CVCL_YK69)

[Text version]
Cell line name U2OS MPX-Nomad
Synonyms MPXNomad; MultiPleXed-Nomad U2OS
Accession CVCL_YK69
Resource Identification Initiative To cite this cell line use: U2OS MPX-Nomad (RRID:CVCL_YK69)
Comments Population: Caucasian.
Characteristics: The green calcium-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboGFP fluorescent protein, a calmodulin-binding peptide fused to a KDEL signal.
Characteristics: The red cAMP-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboFP650 fluorescent protein, a Ht31 peptide fused to a KDEL signal.
Transfected with: UniProtKB; J9PBR7; Entacmaea quadricolor far-red fluorescent protein eqFP650.
Transfected with: UniProtKB; Q6WV12; Pontellina plumata GFP2 (with p.Lys5Glu, p.Val117Met, p.Val149Asp, p.Val151Asp, p.Ala155Thr, p.Phe168Ser and p.Leu200Asp = TurboGFP).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations
  • Mutation; HGNC; 9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
Children:
CVCL_YK70 (U2OS MPX-Nomad ETBR)CVCL_YK71 (U2OS MPX-Nomad NK2R)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Publications

PubMed=29412765; DOI=10.1177/2472630318754828
Mella R.M., Kortazar D., Roura-Ferrer M., Salado C., Valcarcel M., Castilla A., Villace P.
Nomad biosensors: a new multiplexed technology for the screening of GPCR ligands.
SLAS Technol. 23:207-216(2018)

Cross-references
Encyclopedic resources Wikidata; Q98133757
Entry history
Entry creation19-Dec-2019
Last entry update05-Oct-2023
Version number8