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Cellosaurus U2OS-LacO-I-SceI-TetO (CVCL_ZC51)

[Text version]
Cell line name U2OS-LacO-I-SceI-TetO
Accession CVCL_ZC51
Resource Identification Initiative To cite this cell line use: U2OS-LacO-I-SceI-TetO (RRID:CVCL_ZC51)
Comments Population: Caucasian.
Characteristics: Cell line designed to probe the role of chromatin condensation in DNA damage response (DDR) using a lac-repressor/operator-tethering system (Kerafast=ENH105-FP).
Characteristics: Contains 2 integrated copies of a construct that consist of a I-Scel endonuclease cut site (I-SceIcs) sandwiched between 256 copies of the lac operator (lacO) and 96 copies of the tet operator (tetO) (Kerafast=ENH105-FP).
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations
  • Mutation; HGNC; 9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Publications

PubMed=25464843; DOI=10.1016/j.celrep.2014.10.060
Burgess R.C., Burman B., Kruhlak M.J., Misteli T.
Activation of DNA damage response signaling by condensed chromatin.
Cell Rep. 9:1703-1717(2014)

Cross-references
Cell line collections (Providers) Kerafast; ENH105-FP
Encyclopedic resources Wikidata; Q98133788
Entry history
Entry creation02-Jul-2020
Last entry update05-Oct-2023
Version number6