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Cellosaurus N27-A (CVCL_ZJ56)

Cell line name N27-A
Accession CVCL_ZJ56
Resource Identification Initiative To cite this cell line use: N27-A (RRID:CVCL_ZJ56)
Comments Characteristics: Improved in vitro model of dopamine neurons for research on Parkinson's disease. Expresses TH and DAT at 4 times the level of the parent cell line. Also expresses VMAT2, dopaminergic transcription factors, dopaminergic storage protein (PubMed=27512998).
Doubling time: ~36 hours (PubMed=27512998).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [Note=pSV3-neo].
Derived from site: In situ; Embryonic brain, mesencephalon; UBERON=UBERON_0009616.
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Hierarchy Parent: CVCL_D584 (N27)
Sex of cell Sex unspecified
Age at sampling 12FD
Category Transformed cell line
Publications

PubMed=27512998; DOI=10.1371/journal.pone.0160847
Gao L., Zhou W.-B., Symmes B., Freed C.R.
Re-cloning the N27 dopamine cell line to improve a cell culture model of Parkinson's disease.
PLoS ONE 11:E0160847-E0160847(2016)

Cross-references
Cell line collections (Providers) Millipore; SCC196
Encyclopedic resources Wikidata; Q98127400
Entry history
Entry creation02-Jul-2020
Last entry update05-Oct-2023
Version number6