Cell line name | Tango CHRM2-bla U2OS |
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Accession | CVCL_ZK05 |
Resource Identification Initiative | To cite this cell line use: Tango CHRM2-bla U2OS (RRID:CVCL_ZK05) |
Comments | Population: Caucasian. Characteristics: The target GPCR is linked to a TEV protease cleavage site and a GAL4-VP16 transcription factor. When activated the GPCR recruits the beta-arrestin/TEV protease fusion protein, the TEV protease cleaves off the transcription factor that translocates to the nucleus to activate the expression of the bla gene. Characteristics: Stably expresses a beta-arrestin/TEV protease fusion protein and a beta-lactamase reporter gene under the control of an upstream activator sequence (UAS). Transfected with: HGNC; 1951; CHRM2. Transfected with: HGNC; 712; ARRB2. Transfected with: UniProtKB; P04386; Yeast GAL4 (DNA-binding domain). Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). Transfected with: UniProtKB; P04517; Tobacco etch virus (TEV) protease (AA 2038-2279). Transfected with: UniProtKB; P62593; Escherichia coli beta-lactamase bla (with p.Met1_Phe22del, p.Ala23Met, p.Val82Ile and p.Met184Val). Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979. |
Sequence variations |
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Disease | Osteosarcoma (NCIt: C9145) Osteosarcoma (ORDO: Orphanet_668) |
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
Hierarchy | Parent: CVCL_ZJ93 (Tango GPCR-bla U2OS) |
Sex of cell | Female |
Age at sampling | 15Y |
Category | Cancer cell line |
Web pages | https://www.thermofisher.com/order/catalog/product/K1564 |
Cross-references | |
Encyclopedic resources | Wikidata; Q98133096 |
Entry history | |
Entry creation | 02-Jul-2020 |
Last entry update | 05-Oct-2023 |
Version number | 7 |