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Cellosaurus HEK293T HA-PEROXO (CVCL_ZM07)

[Text version]
Cell line name HEK293T HA-PEROXO
Synonyms HEK-293T HA-PEROXO
Accession CVCL_ZM07
Resource Identification Initiative To cite this cell line use: HEK293T HA-PEROXO (RRID:CVCL_ZM07)
Comments Characteristics: Expresses PEROXO-Tag, a chimeric protein consisting of three HA epitopes attached to the N-terminus of mEGFP and a segment of PEX26 fused to the EGFP C-terminus. This tag allows for localization to and insertion into the peroxisomal membrane.
Transfected with: HGNC; 22965; PEX26 (with p.Met1_Val236del).
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [Note=tsA].
Omics: Quantitative peroxisome proteome analysis by proteomics.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0063 (HEK293T)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=32417403; DOI=10.1016/j.isci.2020.101109
Ray G.J., Boydston E.A., Shortt E., Wyant G.A., Lourido S., Chen W.W., Sabatini D.M.
A PEROXO-Tag enables rapid isolation of peroxisomes from human cells.
IScience 23:101109.1-101109.18(2020)

Cross-references
Encyclopedic resources Wikidata; Q98126676
Proteomic databases PRIDE; PXD018880
PRIDE; PXD018918
Entry history
Entry creation02-Jul-2020
Last entry update30-Jan-2024
Version number7