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Cellosaurus T-47D HA-PEROXO (CVCL_ZM09)

[Text version]

Cell line name T-47D HA-PEROXO
Synonyms T47D HA-PEROXO
Accession CVCL_ZM09
Resource Identification Initiative To cite this cell line use: T-47D HA-PEROXO (RRID:CVCL_ZM09)
Comments Characteristics: Expresses PEROXO-Tag, a chimeric protein consisting of three HA epitopes attached to the N-terminus of mEGFP and a segment of PEX26 fused to the EGFP C-terminus. This tag allows for localization to and insertion into the peroxisomal membrane.
Transfected with: HGNC; 22965; PEX26 (with Met1_Val236del).
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Sequence variations Heterozygous for PIK3CA p.His1047Arg (c.3140A>G) (ClinVar=VCV000013652) (from parent cell line).
Homozygous for TP53 p.Leu194Phe (c.580C>T) (from parent cell line).
Disease Invasive ductal carcinoma, not otherwise specified (NCIt: C4194)
Derived from metastatic site: Pleural effusion.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0553 (T-47D)
Sex of cell Female
Age at sampling 54Y
Category Cancer cell line
Publications

PubMed=32417403; DOI=10.1016/j.isci.2020.101109
Ray G.J., Boydston E.A., Shortt E., Wyant G.A., Lourido S., Chen W.W., Sabatini D.M.
A PEROXO-Tag enables rapid isolation of peroxisomes from human cells.
IScience 23:101109-101109(2020)

Entry history
Entry creation02-Jul-2020
Last entry update02-Jul-2020
Version number1