Home  |  Contact

Cellosaurus HEK293 Actin-cpstFRET-Actin (CVCL_ZV87)

[Text version]

Cell line name HEK293 Actin-cpstFRET-Actin
Synonyms HEK293 AcpA; Beta-M-HEK
Accession CVCL_ZV87
Resource Identification Initiative To cite this cell line use: HEK293 Actin-cpstFRET-Actin (RRID:CVCL_ZV87)
Comments Characteristics: Used to report forces in actin in living cells. Has one actin molecule attached on each end of cpstFRET. The cpstFRET (circularly permuted stretch sensitive FRET) sensor consists of a pair of tandemly connected circularly permutated CFP/YFPs (cpCerulean/cpVenus). Mechanical loading twists the dipole orientation from near parallel to diagonal, decreasing FRET efficiency.
Transfected with: HGNC; 132; ACTB.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0045 (HEK293)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=25422450; DOI=10.1073/pnas.1411683111
Guo J., Wang Y.-X., Sachs F., Meng F.-J.
Actin stress in cell reprogramming.
Proc. Natl. Acad. Sci. U.S.A. 111:E5252-E5261(2014)

Cross-references
Cell line collections Kerafast; EBU022
Entry history
Entry creation02-Jul-2020
Last entry update02-Jul-2020
Version number1