ID   HEK293 Actin-cpstFRET-Actin
SY   HEK293 AcpA; Beta-M-HEK
DR   Kerafast; EBU022
RX   PubMed=25422450;
CC   Characteristics: Used to report forces in actin in living cells. Has one actin molecule attached on each end of cpstFRET. The cpstFRET (circularly permuted stretch sensitive FRET) sensor consists of a pair of tandemly connected circularly permutated CFP/YFPs (cpCerulean/cpVenus). Mechanical loading twists the dipole orientation from near parallel to diagonal, decreasing FRET efficiency.
CC   Transfected with: HGNC; 132; ACTB.
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
OX   NCBI_TaxID=9606; ! Homo sapiens
HI   CVCL_0045 ! HEK293
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 02-07-20; Last updated: 02-07-20; Version: 1
RX   PubMed=25422450; DOI=10.1073/pnas.1411683111;
RA   Guo J., Wang Y.-X., Sachs F., Meng F.-J.;
RT   "Actin stress in cell reprogramming.";
RL   Proc. Natl. Acad. Sci. U.S.A. 111:E5252-E5261(2014).