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Cellosaurus HEK293 Actinin-C-cpstFRET (CVCL_ZV88)

[Text version]

Cell line name HEK293 Actinin-C-cpstFRET
Synonyms HEK293 C-cpstFRET
Accession CVCL_ZV88
Resource Identification Initiative To cite this cell line use: HEK293 Actinin-C-cpstFRET (RRID:CVCL_ZV88)
Comments Characteristics: Has one actinin molecule attached to the C-terminal of cpstFRET, leaving the other end of the probe dangling free and the probe devoid of tension (force-free control). The cpstFRET (circularly permuted stretch sensitive FRET) sensor consists of a pair of tandemly connected circularly permutated CFP/YFPs (cpCerulean/cpVenus). Mechanical loading twists the dipole orientation from near parallel to diagonal, decreasing FRET efficiency.
Transfected with: HGNC; 163; ACTN1.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0045 (HEK293)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line

PubMed=25422450; DOI=10.1073/pnas.1411683111
Guo J., Wang Y.-X., Sachs F., Meng F.-J.
Actin stress in cell reprogramming.
Proc. Natl. Acad. Sci. U.S.A. 111:E5252-E5261(2014)

Cell line collections Kerafast; EBU025
Entry history
Entry creation02-Jul-2020
Last entry update02-Jul-2020
Version number1