Cell line name | HEK293 Actinin-M-cpstFRET |
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Synonyms | HEK293 M-cpstFRET |
Accession | CVCL_ZV89 |
Resource Identification Initiative | To cite this cell line use: HEK293 Actinin-M-cpstFRET (RRID:CVCL_ZV89) |
Comments | Characteristics: Used to report forces in actinin in living cells. Has one actinin molecule with the cpstFRET sensor incorporated between spectrin repeat domains 3 and 4 toward the middle of actinin. The cpstFRET (circularly permuted stretch sensitive FRET) sensor consists of a pair of tandemly connected circularly permutated CFP/YFPs (cpCerulean/cpVenus). Mechanical loading twists the dipole orientation from near parallel to diagonal, decreasing FRET efficiency. Transfected with: HGNC; 163; ACTN1. Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
Hierarchy | Parent: CVCL_0045 (HEK293) |
Sex of cell | Female |
Age at sampling | Fetus |
Category | Transformed cell line |
Publications | PubMed=25422450; DOI=10.1073/pnas.1411683111 |
Cross-references | |
Cell line collections (Providers) | Kerafast; EBU024 |
Encyclopedic resources | Wikidata; Q98126661 |
Entry history | |
Entry creation | 02-Jul-2020 |
Last entry update | 29-Jun-2023 |
Version number | 4 |