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Cellosaurus HEK293 Actinin-M-cpstFRET (CVCL_ZV89)

[Text version]

Cell line name HEK293 Actinin-M-cpstFRET
Synonyms HEK293 M-cpstFRET
Accession CVCL_ZV89
Resource Identification Initiative To cite this cell line use: HEK293 Actinin-M-cpstFRET (RRID:CVCL_ZV89)
Comments Characteristics: Used to report forces in actinin in living cells. Has one actinin molecule with the cpstFRET sensor incorporated between spectrin repeat domains 3 and 4 toward the middle of actinin. The cpstFRET (circularly permuted stretch sensitive FRET) sensor consists of a pair of tandemly connected circularly permutated CFP/YFPs (cpCerulean/cpVenus). Mechanical loading twists the dipole orientation from near parallel to diagonal, decreasing FRET efficiency.
Transfected with: HGNC; 163; ACTN1.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0045 (HEK293)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=25422450; DOI=10.1073/pnas.1411683111
Guo J., Wang Y.-X., Sachs F., Meng F.-J.
Actin stress in cell reprogramming.
Proc. Natl. Acad. Sci. U.S.A. 111:E5252-E5261(2014)

Cross-references
Cell line collections Kerafast; EBU024
Entry history
Entry creation02-Jul-2020
Last entry update02-Jul-2020
Version number1