ID   HEK293 Actinin-M-cpstFRET
AC   CVCL_ZV89
SY   HEK293 M-cpstFRET
DR   Kerafast; EBU024
DR   Wikidata; Q98126661
RX   PubMed=25422450;
CC   Characteristics: Used to report forces in actinin in living cells. Has one actinin molecule with the cpstFRET sensor incorporated between spectrin repeat domains 3 and 4 toward the middle of actinin. The cpstFRET (circularly permuted stretch sensitive FRET) sensor consists of a pair of tandemly connected circularly permutated CFP/YFPs (cpCerulean/cpVenus). Mechanical loading twists the dipole orientation from near parallel to diagonal, decreasing FRET efficiency.
CC   Transfected with: HGNC; 163; ACTN1.
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Derived from sampling site: Fetal kidney.
OX   NCBI_TaxID=9606; ! Homo sapiens
HI   CVCL_0045 ! HEK293
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 02-07-20; Last updated: 29-10-20; Version: 2
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RX   PubMed=25422450; DOI=10.1073/pnas.1411683111;
RA   Guo J., Wang Y.-X., Sachs F., Meng F.-J.;
RT   "Actin stress in cell reprogramming.";
RL   Proc. Natl. Acad. Sci. U.S.A. 111:E5252-E5261(2014).
//