ID   MDCK Actin-cpstFRET-Actin
AC   CVCL_ZV91
SY   MDCK AcpA
DR   Wikidata; Q98127195
RX   PubMed=25422450;
CC   Characteristics: Used to report forces in actin in living cells. Has one actin molecule attached on each end of cpstFRET. The cpstFRET (circularly permuted stretch sensitive FRET) sensor consists of a pair of tandemly connected circularly permutated CFP/YFPs (cpCerulean/cpVenus). Mechanical loading twists the dipole orientation from near parallel to diagonal, decreasing FRET efficiency.
CC   Transfected with: HGNC; 132; ACTB.
CC   Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
CC   Cell type: Epithelial cell of kidney; CL=CL_0002518.
CC   Breed/subspecies: Cocker Spaniel.
OX   NCBI_TaxID=9615; ! Canis lupus familiaris (Dog)
HI   CVCL_0422 ! MDCK
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 02-07-20; Last updated: 05-10-23; Version: 6
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RX   PubMed=25422450; DOI=10.1073/pnas.1411683111;
RA   Guo J., Wang Y.-X., Sachs F., Meng F.-J.;
RT   "Actin stress in cell reprogramming.";
RL   Proc. Natl. Acad. Sci. U.S.A. 111:E5252-E5261(2014).
//