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Cellosaurus MDCK Actinin-M-cpstFRET (CVCL_ZV93)

[Text version]

Cell line name MDCK Actinin-M-cpstFRET
Synonyms MDCK M-cpstFRET
Accession CVCL_ZV93
Resource Identification Initiative To cite this cell line use: MDCK Actinin-M-cpstFRET (RRID:CVCL_ZV93)
Comments Characteristics: Used to report forces in actinin in living cells. Has one actinin molecule with the cpstFRET sensor incorporated between spectrin repeat domains 3 and 4 toward the middle of actinin. The cpstFRET (circularly permuted stretch sensitive FRET) sensor consists of a pair of tandemly connected circularly permutated CFP/YFPs (cpCerulean/cpVenus). Mechanical loading twists the dipole orientation from near parallel to diagonal, decreasing FRET efficiency.
Transfected with: HGNC; 163; ACTN1.
Derived from sampling site: Kidney.
Species of origin Canis lupus familiaris (Dog) (Canis familiaris) (NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel.
Hierarchy Parent: CVCL_0422 (MDCK)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=25422450; DOI=10.1073/pnas.1411683111
Guo J., Wang Y.-X., Sachs F., Meng F.-J.
Actin stress in cell reprogramming.
Proc. Natl. Acad. Sci. U.S.A. 111:E5252-E5261(2014)

Cross-references
Cell line collections Kerafast; EBU026
Entry history
Entry creation02-Jul-2020
Last entry update02-Jul-2020
Version number1