|Cell line name||MDCK Actinin-M-cpstFRET|
|Resource Identification Initiative||To cite this cell line use: MDCK Actinin-M-cpstFRET (RRID:CVCL_ZV93)|
|Comments||Characteristics: Used to report forces in actinin in living cells. Has one actinin molecule with the cpstFRET sensor incorporated between spectrin repeat domains 3 and 4 toward the middle of actinin. The cpstFRET (circularly permuted stretch sensitive FRET) sensor consists of a pair of tandemly connected circularly permutated CFP/YFPs (cpCerulean/cpVenus). Mechanical loading twists the dipole orientation from near parallel to diagonal, decreasing FRET efficiency.|
Transfected with: HGNC; 163; ACTN1.
Derived from sampling site: Kidney.
|Species of origin||Canis lupus familiaris (Dog) (Canis familiaris)
(NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel.
|Hierarchy||Parent: CVCL_0422 (MDCK)|
|Sex of cell||Female|
|Age at sampling||Adult|
|Category||Spontaneously immortalized cell line|
|Cell line collections||Kerafast; EBU026|
|Last entry update||02-Jul-2020|