ID   HaCaT-shNrf2#2
AC   CVCL_ZW71
DR   Wikidata; Q102114091
RX   PubMed=31319135;
CC   Population: Caucasian.
CC   Knockout cell: Method=shRNA knockdown; HGNC; 7782; NFE2L2.
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.His179Tyr (c.535C>T); ClinVar=VCV000127815; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Arg282Trp (c.843_844CC>TT); Zygosity=Heterozygous (from parent cell line).
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Back, skin, epidermis; UBERON=UBERON_0001068+UBERON_0001003.
CC   Cell type: Keratinocyte; CL=CL_0000312.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0038 ! HaCaT
SX   Male
AG   62Y
CA   Spontaneously immortalized cell line
DT   Created: 29-10-20; Last updated: 29-06-23; Version: 6
//
RX   PubMed=31319135; DOI=10.1016/j.tiv.2019.104602;
RA   Choi M., Park M., Lee S., Lee J.W., Choi W.J., Lee C.;
RT   "Establishment of Nrf2-deficient HaCaT and immortalized primary human
RT   foreskin keratinocytes and characterization of their responses to
RT   ROS-induced cytotoxicity.";
RL   Toxicol. In Vitro 61:104602.1-104602.12(2019).
//