|| Kim K.-H., Chung P.-S., Park H.-M., Rhee C.-S., Park J.-G.|
||Establishment and characterization of cell lines derived from squamous cell carcinoma of the head and neck.|
|| Korean J. Head Neck Oncol. 12:181-187(1996)|
||We have characterized 4 human squamous carcinoma cell lines established
from the larynx and hypopharynx area. All the cell lines were cultured in
RPMI-1640 medium. During the growth they showed monolayer adherence
pattern in culture flask. They showed tonofilament on transmission
electromicroscopy which is characteristic of squamous cell epithelium.
DNA finger-printing using Hinf-1 proved them to be originated from
different beings. Flow cytometric analysis revealed them to show
aneuploidy. Immunohistochemical staining for cytokeratin was done using
CK1, CK8.13, CK19 and CAM5.2 antibody, and produced various patterns of
positivity. All the cell lines showed varying degrees of tumorigenecity in
athymic nude mice when injected subcutaneously, but only
heterotransplanted SNU-1041 cell line showed continuous tumor growth.
Histopathologic findings of the heterotransplanted tumors were identical
to those of the original tumors of patients. This study suggests that
establishment of many different squamous cell lines might bestow great
capability in researches of the head and neck cancer.
|| CVCL_5063; SNU-46 CVCL_5077; SNU-585 CVCL_5105; SNU-899 CVCL_L085; SNU-1041|