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Cellosaurus publication CLPUB00058

Publication number CLPUB00058
Authors Kadin M.E., Cavaille-Col M.W., Sioutos N., Fletcher J.A., Morton C.C., Pastuzak W., Rezuke W., Altman A.J.
Title Childhood Ki-1+ anaplastic large cell lymphoma: establishment and characterization of a new tumor cell line transplantable to SCID mice.
Citation Blood 76 Suppl. 1:354a-354a(1990)
Abstract Ki-1 anaplastic large cell lymphoma (ALCL) is a newly recognized clinicopathologic entity characterized by a distinctive chromosome translocation, t(2;5)(p23;q35), sinus and paracortical infiltration of lymph nodes, frequent extra-nodal disease (especially skin, soft tissue, CI tract and bone), and expression of lymphocyte activation antigens (CD3O, CD25, transferrin receptor, and HLA-DR) by pleomorphic tumor cells. Spontaneous regression of skin lesions is frequently encountered. The peak age incidence of ALCL is childhood and adolescence. A good response to chemotherapy (COMP or D-COMP) with an excellent event-free survival has been reported for most patients with ALCL; however a subset of patients have treatment resistant disease. To better understand the biology of ALCL and to develop new approaches to therapy, we established a cell line from leukemic cells of a 12 year old boy (JB) with treatment resistant disease. The morphology of JB cells consists of a spectrum of small Sezary-like cells and larger immunoblasts including multinucleated Reed-Sternberg like cells. The immunophenotype is that of an activated T-cell: positive for CD2, CD7, Bfl, CD30, CD25, transferrin receptor, CD45, HLA-DR and for the histiocyte-macrophage marker KP-1. JB cells have the t(2;5) and clonal rearrangement of beta chain T-cell receptor genes but germline configuration of immunoglobulin JH gene. The cloning efficiency is 5% and the doubling time is 60 hr in methylcellulose with IMDM and 15% FCS. JB cells were injected intraperitoneally to form a metastasizing tumor in SCID mice. The transplanted tumor maintains the original morphology, immunophenotype and genotype. Tumor fragments have been passaged repeatedly subcutaneously reaching a tumor size of greater than 1 cm in 4-6 weeks. The subcutaneous tumors remain outside the abdominal wall fascia and do not metastasize. Preliminary experiments of tumor directed therapy have been initiated. This cell line should provide a means of designing more effective therapies for treatment-resistant Ki-1 + ALCL.
Cell lines CVCL_H633; JB6 [Human anaplastic large cell lymphoma]