| Publication number |
CLPUB00060 |
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| Authors |
Gramatzki M., Burger R., Trautmann U., Marschalek R., Lorenz H., Hansen-Hagge T.E., Baum W., Bartram C.R., Gebhart E., Kalden J.R. |
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| Title |
Two new interleukin-6 dependent plasma cell lines carrying a chromosomal abnormality involving the IL-6 gene locus. |
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| Citation |
Blood 84 Suppl. 1:173a-173a(1994) |
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| Abstract |
Two plasma cell lines, INA-6 and JK-6, have been initiated and
continuously cultured from two patients with malignant plasma cell
diseases. Both cell lines are EBNA negative and show morphological and
immunophenotypical features of plasma cells. INA-6 expresses the CD39 and
CDw75 antigens, JK-6 is strongty positive with CD38 and CD39 antibodies.
By flow cytometry they were non-reactive with Ia antibodies and B ceil
reagents CD19, CD20, CD21, CD22, and CD24. While INA-6 cells are releasing
kappa light chains only, JK-6 cells produce IgG kappa. Both cell lines
could only be initiated with IL-6 supplemented medium and remained IL-6
responsive throughout continuous culture. INA-6 is strictly dependent on
IL-6. No spontaneously secreted IL-6 was found nor could it be induced by
IL-1beta /TNFalpha stimulation. Molecular analysis with RT-PCR revealed
mRNA for the IL-6 receptor in both lines. No IL-6 mRNA was detectable in
INA-6 cells, while in JK-6 minute amounts were observed. Cytogenetic
analysis of both lines revealed, among other abnormalities, a deletion
(7)(p13). lnterestingly, the 7p deletion affects the location ot the IL-6
gene. ln both cell lines, IL-6 dependent proliferation could be inhibited
by IFNalpha. IFNalpha had growth regulatory effects only on JK-6: while
high concentrations were inhibitory, low IFNalpha amounts were clearly
stimulatory. A wide variety of other cytokines including GM-CSF and IL-11
did not have the capacity to influence proliferation. These plasma cell
lines do not only allow to turther characterize regulatory events in
plasma cell neoplasias but also provide tools to study therapeutic
interventions.
|
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| Cell lines |
CVCL_5209; INA-6 CVCL_M534; JK-6 |
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