| Abstract |
L6TG, a 6-thioguanine resistant derivative of the original L6 line of rat
myoblasts (Yaffe, 1968), is deficient in hypoxanthine phosphoribosyl-
transferase. L6TG which is resistant to 100 micro g/ml chloramphenicol
(CAP) is a mutant isolated from the above mentioned L6TG line. This double
mutant line provides a new selection system in somatic cell hybridization
experiments, since hybrid cells between this mutant and normal, CAP
sensitive, cells can grow in the HAT selective medium containing CAP,
whereas the parental cells die (Sekiguchi, unpublished). We analyzed the
chromosomes of L6TG and L6TGcapr cells by means of our routine air-dry
method with a combined quinacrine mustard and 33258 Hoechst staining.
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