| Publication number |
CLPUB00077 |
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| Authors |
Kadin M.E., Sako D., Morton C., Newcom S.R., Su I.-J. |
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| Title |
Characterization of a neoplastic T cell line from a patient with cutaneous T cell lymphoma and regressing T cell skin lesions. |
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| Citation |
Lab. Invest. 58:45a-45a(1988) |
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| Abstract |
An activated T-cell line has been established as a continuous suspension
culture for more than two years from IL-2 stimulated Sezary-like cells in
the blood of a 46 year old man with erythroderma and regressing T-cell
skin lesions. Cytogenetics of both the cell line and blood mononuclear
cells before culture reveals the same marker chromosomes t(8;9) (p21;p24)
and t(6;13) (q13;q23). A dermatopathic lymph node two years earlier
showed the same t(8;9) and rearrangements of chromosome 6 at q14.
Interestingly 6q13 is a common fragile site. The clonal derivation of the
cell line was confirmed by T-cell receptor (TCR) gene rearrangement
studies showing the same rearrangements of beta-chain genes in the tumor
cell line and mononuclear cells of the peripheral blood. The cultured
cells, which range in appearance from small lymphoid cells to large
multinucleated cells (<10%), express Ki-1 (CD30), Ia (DR), Tac (CD25), T9,
and T11 (CD2) antigens. Absence of T3 (CD3) antigen on the cell surface
was probably due to lack of transcription for TCR beta chain, since mRNA
for TCR alpha and T3-delta, but not TCR beta, was detected in northern
blots. Cell conditioned media contained biological activity for
transforming growth factor (TGF)-beta which promotes anchorage independent
growth of fibroblasts but limits the clonal expansion of T-cells.
Exogenous TGF beta in nanogram amounts surpressed by 20-40% IL-2 dependent
DNA synthesis of the clinically indolent CTCL-line but did not inhibit
growth of L428 cells derived from advanced nodular sclerosing Hodgkin's
disease. These results suggest that TGF-beta may be important in the
growth regulation of cutaneous T-cell lymphomas. We conclude that this new
CTCL-line may be useful for further studies of T-cell growth and
differentiation.
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| Cell lines |
CVCL_H631; Mac-1 CVCL_H632; Mac-2 |
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