| Publication number |
CLPUB00123 |
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| Authors |
Cinatl J., Gussetis E.S., Cinatl J. Jr., Ebener U., Mainke M., Schwabe D., Doerr H.-W., Kornhuber B., Gerein V. |
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| Title |
Differentiation arrest in neuroblastoma cell culture. |
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| Citation |
J. Cancer Res. Clin. Oncol. 116 Suppl. 1:9-9(1990) |
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| Abstract |
The neuroblastoma cell lines SK-N-SH and UKF-NB 1 to 3 were grown in
liquid and semisolid-fibrin Iscove-medium. Cells of the lines SK-N-SH and
UKF-NB-2 grew as aggregates loosely attached to the surface and formed
multiple protrusions. Cells of the lines UKF-NB-1 and 3 grew also in
aggregates but did not form any protrusions. Clonal culture both of the
SK-N-SH and UKF-NB-2 cells in semisolid medium revealed unipolar character
of the protrusions, in which neurofilaments were found by employing
monoclonal antibodies. Clonal cultures both of the UKF-NB-1 and 3 cell
lines revealed sharply delineated cells. It is proposed that the lines
SK-N-SH and UKF-NB-2 arrested their differentiation at the stage of unipolar
neuroblasts and that the lines UKF-NB-1 and 3 arrested their
differentiation at the stage of apolar neuroblasts. Demonstration of a
differentiation arrest in neuroblastoma culture could be of practical and
theoretical importance.
|
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| Cell lines |
CVCL_XA30; UKF-NB-1 CVCL_9902; UKF-NB-2 CVCL_9904; UKF-NB-3 |
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