| Abstract |
A novel human glioma cell-line designated KINGS-1 was estalished in a
tissue culture system from the surgically removed brain tumor tissue of a
67 years-old Japanese male patient with anaplastic astrocytoma. The single
cell suspension was prepared by trypsin treatment for the culture, then
the cells were washed with RPMI-1640 culture medium and the cell
cultivation was initiated in the same culture medium supplemented with 10%
heat-inactivated fetal calf serum, 100U/ml penicillin and 50 mug/ml
streptomycin in the CO2 incubator. After 10 weeks subcultures were made
possible every 4 to 5 days. The established cell-line was designated as
KINGS-1. Cell surface marker analysis of the KINGS-1 cells was performed
by the use of 41 mouse monoclonal antibodies specific to human leukocytes
differentiation antigens. The results revealed that the all anti-T cell,
anti-B cell, anti-CALLA (CD10) and HLA-DR were found to be negative, while
MCS-2 (CD13) recognizing gp150 kDa a specific marker for the
myelomonocytes and HNK-1 reactive with natural killer cells showed
positive reactivity. Immunoperoxidase stainings of the cultured KINGS-1
cells were carried out using anti-GFAP, anti-S-100 protein, and anti-
vimentin antibodies. These antibodies reactive with the KINGS-1 cells as
well as original fresh tumor cells. To investigate the glial cells
proliferation, differentiation and relationship about cell surface
antigens between glial cells and hematopoietic cells, KINGS-1 will be an
useful model in vitro and a tool for better understanding of human glioma.
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