| Publication number |
CLPUB00155 |
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| Authors |
Kobayashi S., Okada S., Inaba H., Hashimoto K., Nakada T. |
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| Title |
Study on growth characteristics and morphological findings of human small cell carcinoma in vitro. |
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| Citation |
Kokenshi 38:103-111(1986) |
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| Abstract |
The tissue specimens from various biopsied sites and tumors obtained at
the time of surgery of 23 patients with small cell lung cancer diagnosed
with cytological findings were cultured primarily from may '82 to april '85
using Terasaki microplates. 19 (82%) of these patients were succeeded to
subculture and to test in vitro sensitivity assay for various drugs. Of
these, 17 (74%) cell strains subcultured for long term over 1 year were
established. The growth characteristics and morphological findings of 15
human small cell carcinoma cell strains and 4 cell lines subcultured over
5 years were investigated under phase contrast microscope. Almost all of
small cell carcinoma cells (86%) in primary culture were observed to grow
as floating colony like cell aggregates, but the cells tend to grow as
attached to the substrates by continuous subculturing for long term. The
growth characteristics of the established small cell carcinoma cell lines
were classified into 3 categories which consisted of epithelial like,
neuritelike or fibroblast like and floating colony like cells. In some
cases of the epithelial like growing cells, the cell morphology was
changed into the view of large cell carcinoma cells, but not in
neuritelike or fibroblast like growing cells.
|
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| Cell lines |
CVCL_B374; S-1 CVCL_4719; S-2 |
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