| Abstract |
From March 1986 to March 1987, 18 pulmonary carcinoma cell lines
comprising 7 squamous, 4 adenocarcinoma, 3 adenosquamous, 3 small cell and
1 large cell anaplastic cell line and a pulmonary metastatic cell line
deribed from colon carcinoma were established in long term passages of
more than 3 years. In particular, since November 1986, we have tried to
culture primarily tissue specimens obtained from all cases operated to
examine success rates of cultivation. For 5 months between November 1986
and March 1987. there were 32 cases who were diagnosed as having pulmonary
carcinoma on histodiagnosis and whose cells were primary cultured. Twelve
(38%) of the 32 cases were succeeded to subculture for long term over 6
months. In 10 (31%) of these, long term passage of more than 3 years and
establishment of cell lines were successful. Success rates of cultivation
for long term over 6 months were 9/29 (31%) in non-small cell lung cancer
(3/15 in adenocarcinoma, 5/13 in squamous, 1/1 in adenosquamous) and 3/3
(100%) in small cell lung cancer. Growth characteristics of these 19 cell
lines were examined.
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