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Cellosaurus publication CLPUB00185

Publication number CLPUB00185
Authors Kakpakov V.T., Gvozdev V.A., Platova T.P., Polukarova L.G.
Title In vitro establishment of embryonic cell lines of Drosophila melanogaster.
Citation Genetika 5:67-75(1969)
Abstract In vitro establishment of embryonic cell lines of Drosophila melanogaster is described. The enriched C15 medium was used for cell culture. The population-doubling time was 24 h; it did not decrease after the 50 passages. Two sublines with female karyotypes were obtained: 1) diploid subline 67j25D, containing 85-90 percent of diploids; 2) aneuploid subline 67j25A, containing 60-70 percent of aneuploids. After the prolonged cultivation of aneuploid subline a shift of the modal class of aneuploids was marked. For the growth of sublines the presence of bovine fetal serum containing non-dialysed thermostable factor is essential. The addition of pupae extract containing the thermolabile non-dialysed factor(s), particularly at the early passages, is necessary for the active growth of cells.
Cell lines CVCL_WY64; 67j25A
CVCL_Z425; 67j25D