| Publication number |
CLPUB00185 |
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| Authors |
Kakpakov V.T., Gvozdev V.A., Platova T.P., Polukarova L.G. |
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| Title |
In vitro establishment of embryonic cell lines of Drosophila melanogaster. |
|---|
| Citation |
Genetika 5:67-75(1969) |
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| Abstract |
In vitro establishment of embryonic cell lines of Drosophila melanogaster
is described. The enriched C15 medium was used for cell culture. The
population-doubling time was 24 h; it did not decrease after the 50
passages. Two sublines with female karyotypes were obtained: 1) diploid
subline 67j25D, containing 85-90 percent of diploids; 2) aneuploid subline
67j25A, containing 60-70 percent of aneuploids. After the prolonged
cultivation of aneuploid subline a shift of the modal class of aneuploids
was marked. For the growth of sublines the presence of bovine fetal serum
containing non-dialysed thermostable factor is essential. The addition of
pupae extract containing the thermolabile non-dialysed factor(s),
particularly at the early passages, is necessary for the active growth of
cells.
|
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| Cell lines |
CVCL_WY64; 67j25A CVCL_Z425; 67j25D |
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