| Abstract |
Prolonged, serially transferred cultures of hemocytes of Malacosoma
disstria Hubner were described recently (Sohi, 1971). Insect hemolymph was
included in the culture medium used for these cells. However, it is very
difficult to collect blood in quantity from most insects, and practically
impossible to get any useable quantities of blood from certain small
insects such as aphids, leafhoppers, whiteflies, mosquitoes and fruit
flies. Therefore, it is necessary to grow insect cell cultures in a
medium lacking insect hemolymph before it would be practical to use them
on a large scale. The present paper describes a cell line of M. disstria
hemocytes obtained with a hemolymph-free medium. The cell line is
designated as IPRI 108.
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