Cellosaurus logo
expasy logo

Cellosaurus publication CLPUB00269

Publication number CLPUB00269
Authors Zhuo J.-C., Wang M.-C., Tao X.-M., Li M., Nie L.-P., Zhang Q.-L., Shi D.-Y.
Title Establishment of arsenic trioxide-resistant K562/AS2 cell line and the mechanism of multidrug resistance.
Citation Chin. J. Pathophysiol. 18:840-843(2002)
Web pages http://www.cjpp.net/EN/Y2002/V18/I7/840
Abstract Aim: To establish a arsenic trioxide (As2O3)-resistant leukemic cell line to explore the mechanism of resistance to As2O3, and the relationship between the resistant cell line and the multidrug resistance was also investigated. Methods: The arsenic trioxide (As2O3)-resistant leukemic cell line was established by exposing the cells to the increasing concentration of As2O3. MTT assay was used to detect the cytotoxicity. Cell cycle was detected by PI assay. Flow cytometry was used to detect the P-glycoprotein on the surface of the cells, the intracellular concentration of DNR, and the immune type of the cells. Results: The cell doublings time and the cell cycle of the arsenic trioxide (As2O3)-resistant leukemic cell line, K562/AS2, is similar to that of K562. The relative resistant fold of K562/AS2 to As2O3, DNR, VP16 and Ara-C was 7.4, 2.9, 3.8 and 1.1, respectively. The relative resistant fold of multidrug resistant cell line, K562/AS2, to As2O3, DNR, VP16 and Ara-C was 0.8, 9.4, 2.5 and 0.9, respectively. The fluorescence of the P-glycoprotein on the surface or of the DNR inside the cells detected was not significantly different between the K562 and the K562/AS2 cell lines. Conclusions: A cell line, K562/AS2, resistant to clinical achieving level (2 mumol/L) of As2O3 has been established. The relative resistant fold of K562/ AS2 to As2O3 is about 7.4 fold to the parent K562 line sensitive to As2O3. Partial resistance of K562/AS2 to DNR and VP16 is observed , the mechanism of which is unrelated to the P-gp, the expression product of multidrug resistance gene 1 (mdr1).
Cell lines CVCL_4V85; K562/AS2