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Cellosaurus publication CLPUB00273

Publication number CLPUB00273
Authors Ginot L., Jeannesson P., Angiboust J.-F., Jardillier J.-C., Manfait M.
Title Interactions of adriamycin in sensitive and resistant leukemic cells: a comparative study by microspectrofluorometry.
Citation Studia Biophys. 104:145-153(1984)
Web pages https://jglobal.jst.go.jp/en/detail?JGLOBAL_ID=200902099133470377
https://eurekamag.com/research/028/559/028559879.php
Abstract Based on the fluorescence properties of adriamycin (ADM), fluor-escence emission spectra of this drug were recorded at the single cell level using a highly sensitive microapectrofluorometer. Spectra were obtained from micrometer regions in the cytoplasm or in the nucleus of single living tumor cells which originated from culture or from mice ascitic tumors. In sensitive ADM treated cells, ADM spectra show that the drug does not interact during its cytoplasmic transit but intercalates in nuclear DNA in a similar way to that obtained when ADM is combined With calf thymus DNA. With ADM resistant leukemic cells, no intracellular fluorescence signal was recorded either in the cytoplasm or in the nucleus which suggests an inhibition of the drug uptake by resistant cells. By increasing extracellular concentration of ADM, resistant cells allowed the drug to penetrate but no signal of free or intercalated drug was observed.
Cell lines CVCL_D573; K562/R7